Best Practices in Microbiota Research
Mikael Niku
Radko Avi
Eveliina Hanski
Antti Karkman
University of Helsinki
26th of September, 12:00 - 15:30 EEST
Zoom link will be sent to registered participants
The "Best Practices in Microbiota Research" webinar brings together leading researchers to share cutting-edge methods and insights in the field of microbiota science. Dr. Mikael Niku will introduce fundamental concepts in microbiota research, as well as advanced techniques like 16S rRNA gene amplicon sequencing. Dr. Eveliina Hanski will cover essential practices for microbiota sample collection, storage, and processing. Dr. Antti Karkman will delve into metagenomic sequencing and its application to studying antimicrobial resistance genes. Dr. Radko Avi will conclude with a discussion on the analysis of the virome from human fecal samples. This comprehensive program is an invaluable resource for researchers, students, and professionals interested in microbiota and virome research.
Webinar Program:
12.05 Introduction
12.10 Dr. Eveliina Hanski: Microbiota Sample Collection, Storage, and Processing
12.50 Dr. Mikael Niku: 16S rRNA Gene Amplicon Sequencing and Data Analysis
13.20 Dr. Antti Karkman: Metagenomic Sequencing and Data Analysis (Including Antimicrobial Resistance Genes)
14.00 Dr. Radko Avi: Analysis of the Virome from Human Fecal Samples
Eveliina Hanski
Post doc researcher & lecturer
Faculty of Medicine, University of Helsinki
Department of Biology & Magdalen College, University of Oxford
Eveliina Hanski is a researcher working on the mammalian gut microbiota, with a focus on its development in early life. Her work focuses on understanding both the temporal changes within the microbial community as well as their influence on host development and health. She has extensive experience in working with a range of systems from lab mouse models to humans and wild animals.
Mikael Niku
Title of Docent, Microscopic Anatomy
Senior University Lecturer, Veterinary Biosciences
University of Helsinki
Mikael Niku is a senior lecturer at Veterinary Biosciences, University of Helsinki. He leads the Developmental Interactions research group, investigating how the maternal microbiota modulates the perinatal development of the intestinal immune system.
Niku studied biochemistry and developmental biology at the University of Oulu. He did his PhD on the development of the bovine immune system and circulating stem cells at the University of Helsinki. He then worked as lecturer in physiology and anatomy at the Department of Human Nutrition, studying the impacts of diet on intestinal health. In 2015, he visited the Veterinary Faculty at Melbourne University.
Niku’s group has studied the early development of the gut microbiota in cattle, horses and pig. Currently he is especially interested in how the metabolites and extracellular vesicles produced by maternal microbiota influence the mammalian fetus. He is actively collaborating with cattle and pig health researchers in Finland and elsewhere. Niku is in the board of the Finnish Society for Immunology.
Antti Karkman
University Lecturer, Department of Microbiology
University of Helsinki
Antti Karkman is a University Lecturer at the Department of Microbiology at the University of Helsinki. He is a HiLIFE fellow and also affiliated with the Helsinki Institute of Sustainability Science (HELSUS) and serves as a supervisor for two doctoral programs: the Doctoral Programme in Food Chain and Health, and the Doctoral Programme in Microbiology and Biotechnology. Antti’s research focuses on microbiology, ecology, and evolutionary biology, with an emphasis on antibiotic resistance.
Radko Avi
Associate Professor, University of Tartu
I have experience in studying HIV-1 drug resistance mutations, particularly within the unique epidemiological context of Estonia’s HIV-1 CRF06_cpx epidemics. My work includes contributing to the creation of the Estonian HIV database and exploring the relationship between human genetic factors—primarily chemokine receptors and their ligands (CCL3L1, CCR5, etc.)—and bloodborne viral infections such as HIV, HBV, HCV, and GBV-C. Since the onset of the COVID-19 pandemic, I have been involved in developing SARS-CoV-2 genotyping assays and mapping the epidemiological landscape in Estonia. Currently, my research focuses on the human gut microbiome and virome.